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Another NGS method is called pyrosequencing, commercialized by 454 Life Sciences. Contrary to Illumina sequencing, this method does not use reversible chain terminators. Instead it uses a special enzyme called luciferase that emits light as specific dNTPs are added. This process is repeated for the 4 dNTPs (similarly to Sanger sequencing) and from the light emissions we can deduce the sequence of nucleotides44.，详情可参考WPS极速下载页
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